Culture and isolation of phototrophic sulfur bacteria from. Isolation and characterization of pah biodegrading marine bacteria. Isolation of typical marine bacteria by dilution culture applied and. The variability of chemical composition and physical conditions in the oceans play a major role in the development of a great diversity of microbes. By a process of double filtration of seawater, first through a membrane filter with a pore diameter of 0. Their theory that these organisms live there at the expense of radioactive radiation rather than of light, has. Marine bacteria were isolated from various sea samples and then they were screened for the production of alkaloids. Actinobacteria actinomycetes have been received much attention, as these bacteria produce a variety of natural drugs and other bioactive metabolites. Marine sedentary organisms marine plants and sessile animals form highly specific and symbiotic relationships with bacteria 8, 9, 10 and in extreme cases up to 60% of the tissue volume 11. Lipases are produced by microorganisms such as bacteria and fungi.
Isolation and screening of marine bacteria producing. More than half of 86 isolates from the southern californian bight showed antagonistic activity and especially bacteria from marine snow displayed strong antagonistic activities towards other bacteria 15. This harbor like others from europe has been chronic contaminated with petroleum. The isolation of marine actinomycetes has been a great source of new compounds and their isolation all around the world from deepest sediments to the shallow costal sediments from the mariana trench, demonstrates that actinomycetes are everpresent in. Studies involving the screening, isolation and characterization of bioactive compounds from marine bacteria are yet to be undertaken on a systematic scale. Polyaromatic hydrocarbons pah are considered to be important and dangerous pollutants which cause serious health problems andor genetic defects in humans, as well as harming the flora and fauna of. The crude extract from bacteria strains cgh2xx was found to have high cytotoxicity against the cancer cell line hl60 ic 50 0. The present study indicated that among 6 marine bacteria associated with sponges showed growth inhibition against indicator microorganism.
Isolation of an algal morphogenesis inducer from a marine. This cell type had the dimensions of ultramicrobacteria. Isolation of spongeassociated bacteria dilution method was applied for the isolation of spongeassociated bacteria. Isolation and characterization of marine sediment bacteria. Two bacteria,one with orange andthe other with yellow pigmentation,were isolated and developed into axenic cultures. Microbiology module methods of isolation of bacteria microbiology 110 notes zmicroscopic examination of sample zvarious methods used for isolation of bacteria specimen collection many different specimens are sent for microbiological examination from patients with suspected bacterial infection. Isolation of predominant bacteria colonies isolate on marine agar or zobell medium the predominant different types observed by taking with a sterile pasteur pipette or a sterile loop a representative colony and spread it on agar. Isolation and cultivation methods of actinobacteria. The samples were subsequently incubated on one general and six selective agar media of varying compositions of nutrients to enrich mainly for bacteria of more. Ramana3 1department of biotechnology, college of science and technology, andhra university,visakhapatnam 530003, 2department of biotechnology, visakha government degree. The isolation of bacteria from sewage water uk essays. Evaluation of screening methods for the isolation of biosurfactant producing marine bacteria.
Populations are measured, diluted to a small and known number of cells, inoculated into unamended sterilized seawater, and examined three times for the presence of 104 or more cells per ml over a 9week interval. The isolation of this actinomycete is difficult because of the fast growth of fungi and other bacteria in the test samples in isolation plate with lower ph. A phylogenetic tree analysis of 91 sequences identified the genus or species level was carried out. The isolation and identification of brucella can confirm a diagnosis of brucellosis. The populations present before dilution varied from 0. Isolation of marine bacteria, antagonistic to human pathogens. Marine bacteria provide a useful source for bioremediation and have an advantage over terrestrial bacteria because they can be used in extreme environmental conditions. Isolation of marine bacteria with antimicrobial activities. Jul 12, 2018 the present study deals with the isolation and screening of marine bacteria for biofilm formation. If a laboratory is not available to perform culture and isolation, please contact your state health department for assistance. Seawater sample was collected from constanta harbor romania, which is the largest harbor on the black sea and one of the largest ports in europe.
Research article selective screening, isolation and characterization of antimicrobial agents from marine actinomycetes y. Marine sediments from south east coast of bay of bengal were selected as a potential source of marine actinomycetes and possible bioactivity. Isolation of marine bacteria with antiviral properties. Mar 11, 2005 ulva and enteromorpha are cosmopolitan and familiar marine algal genera. Pdf isolation of marine bacteria with antimicrobial activities from. The presence and isolation of gramnegative bacteria is common in marine samples, especially shrimp residues. This study is focused on the isolation of different species of marine bacteria on marine agar from different coastal water sources, production of. Degradation of plastics and plasticdegrading bacteria in. In c14 gaseous hydrocarbons, both strains grew only on ethylene, but degraded ethane, propylene, and propane in. From 101 marine samples, 166 pigmented bacteria were isolated.
Yim 6, yim 17, yim 21, and yim 47 media can be used for isolating acidophilic actinomycetes. In these experiments, we isolated marine bacteria that have significant antiviral activity against the human enterovirus, poliovirus type 1 sabin lsc2ab, from several sites around the island of oahu. Antibacterial activity of marine bacteria isolated from. Gramnegative bacteria are the cause of diseases in crustaceans, which mainly attack the cuticle of these organisms sharmila et al. A presentation of msc thesis on the isolation, identification and characterization of cellulose degrading bacteria from lake and river in england. Marine bacteria were isolated from water samples obtained from kerala sea coast in india. Button, 1, frits schut, 2 pham quang, 3 ravonna martin, 1 and betsy r. Antiviral activity in coastal marine waters was removed by. Rm42 had broad spectral antimicrobial activity, and were selected for further studies, and they represented.
These tissues are monitor organs that usually harbor the diseasecausing bacteria during infection. Isolation and characterization of marine antagonistic actinomycetes from west coast of india 15 both e. The distribution of actinomycetes in various natural habitats, including soil, ocean, extreme environments, plant, lichens and animals, is described. This material was suspended and homogenized in 9 ml 0. Cultivation of bacteria protocols for use of cultivation of bacteria, use of general growth, enriched, selective and.
The present studies of the goals of this paper were isolation. Students learn and understand the significance of bacterial isolation in applied biotechnology. The most widely studied microbial species from the indian coastal waters as a source of antibiotics has. Pdf isolation of broadhostrange replicons from marine. As noted previously, once plastic wastes enter the marine environment, the large particles of plastic are first fragmented to form microplastics or nanoplastic particles debroas. The collection and pretreatment of test samples from different sources, design principle of. Antimicrobial activity of marine bacteria associated with. Apr 12, 2018 the production of commercial enzymes, including lipase from bacteria has always been the industrial choice due to its economical and commercial feasibility. It is well known that these green macroalgae lose their natural morphology during shortterm cultivation under aseptic conditions and during longterm cultivation in nutrientadded seawater and adopt an unusual form instead. Viability and isolation of marine bacteria by dilution. Isolation and characterization of pah biodegrading marine bacteria sulaiman ali alharbi 1, m. Antimicrobial and antioxidant activities of bacterial. Isolation and characterization of bioactive compounds from. Microbiology module methods of isolation of bacteria microbiology 110 notes zmicroscopic examination of sample zvarious methods used for isolation of bacteria specimen collection many different specimens are sent for microbiological examination from.
Therefore, isolation of bacteria and functional screening intended for salt stress tolerant property, characterization and molecular classification of the marine salt. One gram sponge sample was cleaned using sterile sea water to remove the nonattached bacteria. Isolation and screening of marine bacteria producing antibiotics against human pathogens article in international journal of pharma and bio sciences 24. When sending specimens to a laboratory for culture and isolation, please be sure the laboratory is aware of your suspected diagnosis. Our results demonstrate that the marine bacteria from corals have great potential in the discovery of useful medical molecules.
Isolation and characterization of marine bacteria from east coast of. In the pouring method, there is an overgrowth of bacterial colonies from which the isolation of pure culture is very difficult. First filter out the particulate matter with a 20 um filter, then you can use water sampling nitrocellulose filter disc of 0. Viability and isolation of marine bacteria by dilution culture. Bacterial isolates 340 and counting were recovered from the cultures, and the 16s rdna of 96 strains was sequenced. Our study demonstrated that marine purple photosynthetic. These distinctive characteristics have attracted many researchers to explore in depth since there is. Faculty of natural resources and agricultural sciences uppsala biocenter. Pdf isolation, screening and identification of marine. Various pretreatment procedures and selective media were applied to assess the optimal conditions for the isolation of marine actinomycetes from sediments. Pdf isolation and identification of marine bacteria from. The following procedure and media are from our old bacteriology 320 lab manual circa 1984, and isolation was generally successful when 1020 enrichments were set up initially. As the bacteria form biofilms, there is a greater increase in their bioremediation capability due to the presence of eps like polysaccharides, amyloids, extracellular enzymes.
Alteromonas was the dominant antagonistic marine bacteria, exhibiting a wide antibacterial spectrum against the human pathogens associated with seafoods such. M kawarau, i pratiwi, a sunudin 2017 isolation and characterization of marine bacteria from macro algae gracillaria salicornia and gelidium latifolium on agarolytic activity for bioethanol production. Various food samples procured from local markets of kashmir were used in this studyto isolate pigment producing bacteria. Biofouling is considered as a main issue of concern in aquatic environment causing severe economic loss and pollution. This requires separation techniques that allow isolation of a pure culture of one type of bacteria. Methods of isolation of bacteria microbiology module microbiology notes 10 methods of isolation of bacteria 10. Isolation and characterization of filterable marine bacteria. Isolation and screening for secondary metaboliteproducing bacteria sponges have been strongly neglected until now in comparison with the invertebrate parts. Isolation and screening of marine bacteria producing anti. Isolation and identification of marine bacteria producing. In scottish coastal waters 35% of surfaceassociated. Marine bacteria in resurrection bay near seward, alaska, and in the central north sea off the dutch coast were cultured in filtered autoclaved seawater following dilution to extinction. Isolation of two novel marine ethyleneassimilating.
We used serial dilution technique for isolation of marine bacteria, streak plate technique for screeninig of priliminery antibacterial. Pigmented marine bacteria were enumerated for the isolation of antagonistic marine bacteria. The production of commercial enzymes, including lipase from bacteria has always been the industrial choice due to its economical and commercial feasibility. Production of lasparaginase was carried out in submerged fermentation. Isolation and cultivation methods of actinobacteria intechopen. Isolation and characterization of new marine oildegrading. However, we have focused on bacterial microbial lipases were economically importance of several properties. In the isolation of the bacteria, either way for a dilute sample such as water you would probably have to concentrate the sample. Bacteriology 102 isolation of marine bioluminescent bacteria. Isolation and characterization of the first phage infecting. Isolation, characterization and identification of an. Dilution culture, a method for growing the typical small bacteria from natural aquatic assemblages, has been developed. The aim of the present study was to isolate marine fungus antagonistic to biofouling bacteria and to define antifouling compounds.
Production of lasparaginase was carried out in submerged fermentation media. This study is focused on the isolation of different species of marine bacteria on marine agar from different coastal water sources, production of natural. This technique is least preferred for the separation of pure culture. Isolation of bacteria can define as the technique of separating one species of bacteria from the mixed culture of bacteria by different plating methods like pouring, spreading, streaking and serial dilution. Isolation of typical marine bacteria by dilution culture. The microhabitats for marine bacteria are seawater, sediments, animate and inanimate surfaces. Isolation of predominant bacteria in marine molluscs. In a previous study, we evaluated the potential use of marine purple photosynthetic bacteria for pha production higuchitakeuchi et al. Isolates may be sent to cdc for identification and molecular characterization. Metabolic and ultrastructural response to glucose of two eurytrophic bacteria isolated from seawater at different enriching concentrations. The characteristics of both strains were investigated, and phylogenetic analyses of their 16s rrna gene sequences showed that they belonged to the genus haliea.
Twentyfive grams of agar for 1 l should be used, and ph should be adjusted to 4. The objective of this investigation was to isolation and screening of marine bacteria for lasparaginase activity. In general, for the isolation of obligate marine actinomycetes from different marine sources, low nutrient media work better than nutrientrich media olson et al. Totally, 35 morphologically different microorganisms were isolated and were screened for. Pdf isolation of antagonistic marine bacteria from the. The soil samples were collected aseptically and subjected to serial dilution to isolate the bacterial strains.
Pdf isolation and screening of marine bacteria and. Isolation and characterization of marine biofilm forming. Isolation strategies of marinederived actinomycetes from. The isolates were identified as marine bacteria by microscopical and biochemical tests. Brucella is most commonly isolated from blood cultures. These phenomena led to the belief that undefined morphogenetic factors that were indispensable to. Naturally occurring plasmids isolated from heterotrophic bacterial isolates originating from coastal california marine sediments were characterized by analyzing their incompatibility and replication properties. By popular demand, here is a procedure for the isolation of bioluminescent bacteria from a couple reliable source materials, namely shrimp and squid. The gram staining method was first described in 1844 by the danish bacteriologist hans christian gram, after whom the test was. Two novel ethyleneassimilating bacteria, strains etym and etynag, were isolated from seawater around japan. Aim of this study deals with screening and characterization of chitinaseproducing bacteria from marine waste and its deposited soil along the coastal regions in chennai. Some marine bacteria secrete exopolysaccharides 16 and many marine freeliving bacteria produce secondary metabolites possessing antibacterial properties 17. Culture and isolation of phototrophic sulfur bacteria from the marine environment. Isolation of histamineproducing bacteria from frozen tuna.
The surfaces and internal spaces of these organisms are. Isolating microorganisms from marine and marineassociated. The screened isolates were characterized and identified as pychrobacter celer, pychrobacter alimentarius and kocuria rhizophila by 16s rrna sequencing. Biodegradation is a process by which microbial organisms mainly bacteria and fungi transform or alter through metabolic or enzymatic action the structure of chemicals introduced into the environment muthu 2014. Isolates of these ultramicrobacterial cultures that were eventually obtained on relatively high nutrient agar. Isolation and characterization of new marine oildegrading bacteria romanian biotechnological letters, vol. Ten grams of collected sediments was serially diluted using sterile phosphate buffer in 10folds and the 105. The m that showed positive result for the production of alkaloids were then subjected to study under varying physical and chemical conditions namely.
Bacteria are usually isolated from fish kidney and spleen. Pdf bacteria associated with eight fieldcollected and five cultured soft corals of briareum sp. Three different pretreatment methods were employed. Pdf isolation and characterization of a marine bacterium from. Isolation and screening of marine bacteria and actinomycetes for enzyme production and antibacterial activity from vadinar coast, jamnagar, gulf of kachchh january 2015 bhavini s ganatra. Spreading method is again a very simple method to perform isolation of bacteria. Isolation of antagonistic marine bacteria from the surface of the gorgonian corals at tuticorin, south east coast of india. Each of 11 experimental trials of the technique was successful. Isolation and characterization of a marine bacterium from sundarbans, bangladesh article pdf available february 2015 with 553 reads how we measure reads. Pdf isolation and characterization of a marine bacterium. Populations are measured, diluted to a small and known number of cells, inoculated into unamended sterilized seawater, and examined three times for the presence of 104 or more cells per ml over a 9. In simple words, isolation of bacteria can define as the method of obtaining a pure culture which is devoid of any contamination and. The pigmented bacterial population was lower by about 23 log counts than the total culturable bacterial population fig. Therefore, it is necessary to develop a screening method for the isolation of phaproducing marine purple nonsulfur photosynthetic bacteria.
Following isolation of bacteria, students characterize the bacteria with household disinfectant products and antibiotics. Marine sediments were collected from irodong, mokposi, jeollanamdo, south korea latitude. Get a printable copy pdf file of the complete article 2. Isolation is done to obtain pure bacterial cultures. Evaluation of screening methods for the isolation of.946 53 417 300 1519 1298 589 922 562 113 1308 1513 886 1215 1459 544 427 296 45 120 157 1573 198 57 174 776 614 657 945 562 1308 601 1438 65 456 501